Shellab BACTRON600 Manual do Utilizador

BACTRON® 110 – 120 Volts Installation and Operation Manual BACTRON300, BACTRON600, BACTRON900 Previously Designated: BACTRONII, BACTR

10 | P a g e GRAPHIC SYMBOLS (CONTINUED) Symbol Definition WEEE Directive compliant logo Indicates the unit should be recycled (Not disposed of

11 | P a g e INSTALLATION AMBIENT CONDITIONS This workstation is intended for use indoors, at room temperatures between 15C and 30C (59F and

12 | P a g e INSTALLATION (CONTINUED) HIGH ALTITUDE LOCATIONS BACTRON anaerobic workstations are equipped with two (2) digital vacuum controller

13 | P a g e INSTALLATION (CONTINUED) LIFTING AND HANDLING The BACTRON is heavy, and care should be taken to use appropriate lifting devices tha

14 | P a g e INSTALLATION (CONTINUED) GAS SOURCE Warning: Never exceed a 5% hydrogen concentration inside the unit’s chamber. Avertissement:

15 | P a g e INSTALLATION (CONTINUED) BACTRON900 ORIENTATION BACTRON600 ORIENTATION Gas and Vacuum Ports Main Control Panel Manometer S

16 | P a g e INSTALLATION (CONTINUED) BACTRON300 ORIENTATION Manometer Main Control Panel Pass Box Arm Ports Workspace Chamber Incubator C

17 | P a g e INSTALLATION (CONTINUED) WORKSPACE CHAMBER ORIENTATION (ALL UNITS) Figure 1: Workspace Chamber Layout PASS BOX ORIENTATION Figure 2

18 | P a g e INSTALLATION (CONTINUED) CLEANING The BACTRON interior was cleaned and disinfected at the factory, but not sterilized. See the Cleani

19 | P a g e CONTROL PANEL OVERVIEW Figure3: Main Control Panel Main Control Panel BACTRON300 The main control panel on the BACTRON300 controls t

2 | P a g e BACTRON Anaerobic Workstation 110 - 120 Volts Installation and Operation Manual Part number (Manual): 4861700-1 Revision: August 27,

20 | P a g e CONTROL PANEL OVERVIEW (CONTINUED) Main Control Panel Continued Power Switch The main power switch on the control panel controls al

21 | P a g e CONTROL PANEL OVERVIEW (CONTINUED) Pass Box Doors Light The clear Doors pilot light indicates that both pass box doors are closed. Th

22 | P a g e CONTROL PANEL OVERVIEW (CONTINUED) Automatic Start / Reset Switch The all black Automatic Start / Reset switch initiates or aborts the

23 | P a g e CONTROL PANEL OVERVIEW (CONTINUED) Workspace Incubator Control Panel (BACTRON900 / BACTRONIV-900 Only) Figure 5: BACTRON900 Workspac

24 | P a g e OPERATION THEORY OF OPERATION AND MAJOR COMPONENT FUNCTIONS Achieving Anaerobic Conditions No OxygenHydrogenPositive PressureActive

25 | P a g e OPERATION (CONTINUED) Accessing the Workstation Items such as media containers and laboratory equipment can be introduced to or rem

26 | P a g e OPERATION (CONTINUED) The Over Temperature Limit System The OTL is a mechanical heating control backup system included with each incu

27 | P a g e OPERATION (CONTINUED). SETTING UP THE BACTRON Perform the following steps to set up the BACTRON workstation for use: 1. Remove all pr

28 | P a g e OPERATION (CONTINUED). 7. Perform the following procedures to setup the unit for use. Setup the Gas Supply page 29 Single Gas Conne

29 | P a g e OPERATION (CONTINUED) Figure 12: Gas and Vacuum Ports SET UP THE GAS SUPPLY See the Gas Source entry in the Installation section on p

3 | P a g e TABLE OF CONTENTS INTRODUCTION ...

30 | P a g e OPERATION (CONTINUED) SINGLE GAS CONNECTION Follow these steps to connect a single cylinder of anaerobic mixed gas. 1. Locate the T-

31 | P a g e OPERATION (CONTINUED) CONNECTING TO AN IN-HOUSE VACUUM SYSTEM Carry out this procedure to connect the BACTRON to an in-house vacuum sy

32 | P a g e OPERATION (CONTINUED) CONNECTING THE FOOT PEDAL ASSEMBLY 1. Place the foot pedal assembly on the floor below the arm port doors. 2.

33 | P a g e OPERATION (CONTINUED) SHELVING INSTALLATION BACTRON300 AND BACTRON900 Install the three (3) metal bottom spacers included with the B

34 | P a g e OPERATION (CONTINUED) ESTABLISHING AN ANAEROBIC ENVIRONMENT Carry out the following steps to purge the aerobic atmosphere from the wo

35 | P a g e OPERATION (CONTINUED) Establishing an Anaerobic Environment Continued Note: If you have opened the left armor port door to install t

36 | P a g e OPERATION (CONTINUED) INSTALLING THE SLEEVE ASSEMBLIES Prior to verifying the achievement of an anaerobic atmosphere, install the

37 | P a g e OPERATION (CONTINUED) CHAMBER ENTRY Perform these steps to access and work in the workspace chamber without drawing in aerobic atmo

38 | P a g e OPERATION (CONTINUED) VERIFYING AN ANAEROBIC ATMOSPHERE This procedure verifies that an anaerobic atmosphere has been established pr

39 | P a g e OPERATION (CONTINUED) TROUBLESHOOTING THE PRESENCE OF OXYGEN 1. Verify that proper sleeve donning, entry, and exiting procedures

4 | P a g e Reactivating the Catalyst Cartridge...

40 | P a g e OPERATION (CONTINUED) EXITING THE CHAMBER The following steps are used to exit the chamber without pulling in aerobic atmosphere.

41 | P a g e OPERATION (CONTINUED) SETTING THE INCUBATOR TEMPERATURE 1. Using a coin or other flat metal implement, turn the Over Temperature L

42 | P a g e OPERATION (CONTINUED) OPERATING THE PASS BOX The pass box is used to introduce items into or remove items from the workspace chamber

43 | P a g e OPERATION (CONTINUED) Manual Cycle The manual cycle is intended as a backup for use in the event the auto cycle is not functioning.

44 | P a g e OPERATION (CONTINUED) LOADING THE WORKSTATION Sheldon Manufacturing recommends waiting 24 hours after establishing an anaerobic a

45 | P a g e OPERATION (CONTINUED) REACTIVATING THE CATALYST CARTRIDGE An active catalyst cartridge must be installed to maintain anaerobic c

46 | P a g e OPERATION (CONTINUED) CALIBRATE THE INCUBATOR TEMPERATURE DISPLAY Temperature calibrations are performed periodically to ensure that

47 | P a g e OPERATION (CONTINUED) 8. Allow the incubator a minimum of four (4) hour to stabilize after reaching the new temperature. 9. Compa

48 | P a g e OPERATION (CONTINUED) ACTIVATED CHARCOAL SCRUBBER AND REJUVENATION CYCLE An activated charcoal scrubber can be placed in the work

49 | P a g e OPERATION (CONTINUED) DEIONIZED AND DISTILLED WATER Note: Do not use deionized water for cleaning or humidifying your incubator. U

5 | P a g e INTRODUCTION Thank you for purchasing a Shel Lab BACTRON Anaerobic Chamber Workstation. We know that in today’s competitive marketplace

50 | P a g e USER MAINTENANCE Chamber Quality Control Check Sheet Month: Temperature Catalyst Changed Condensate Drained Cylinder Pressur

51 | P a g e USER MAINTENANCE (CONTINUED) DAILY MAINTENANCE 1. Exchange the catalyst cartridge with a reactivated one. 2. Remove and empty the

52 | P a g e USER MAINTENANCE (CONTINUED) LEAK DIAGNOSIS – UNIT FULL Perform this leak check procedure for a unit that is in use and that cannot be

53 | P a g e USER MAINTENANCE (CONTINUED) LEAK CHECK – EMPTY UNIT Use this procedure to verify the atmospheric integrity of a BACTRON that is not i

54 | P a g e USER MAINTENANCE (CONTINUED) 11. A gas leak detector capable of detecting hydrogen (Part Number 4600501) can be used to find leaks al

55 | P a g e USER MAINTENANCE (CONTINUED) REPLACING THE SLEEVE CUFFS A sleeve cuff should be replaced if it shows signs of brittleness or dryness

56 | P a g e USER MAINTENANCE (CONTINUED) Warning: Disconnect the power cord from the wall power supply prior to cleaning or disinfecting the BACTR

57 | P a g e USER MAINTENANCE (CONTINUED) DISINFECTING Disinfect the BACTRON on a regular basis. Perform the following steps to disinfect the w

58 | P a g e USER MAINTENANCE (CONTINUED) MAINTAINING THE ACRYLIC GLASS PANELS Sheldon Manufacturing recommends using Novus brand acrylic glas

59 | P a g e USER MAINTENANCE (CONTINUED) CONDENSATION AND THE DEW POINT Relative humidity inside the BACTRON should never exceed 80% at 25°C. Ex

6 | P a g e INTRODUCTION (CONTINUED) ENGINEERING IMPROVEMENTS Sheldon Manufacturing continually improves all of its products. As a result, enginee

60 | P a g e UNIT SPECIFICATIONS These BACTRONs are110 - 120 workstations. Please refer to the BACTRON’s data plate for individual electrical speci

61 | P a g e UNIT SPECIFICATIONS (CONTINUED) STAND DIMENSIONS Model Inches W × D × H Centimeters W × D × H BACTRON300 (BACTRONII) 61.5 x 30 x 30 in

62 | P a g e UNIT SPECIFICATIONS (CONTINUED) CAPACITY Workspace Chamber Volume Model Cubic Feet Cubic Liters BACTRON300 (BACTRONII) 17.6 498 BACTR

63 | P a g e UNIT SPECIFICATIONS (CONTINUED) TOTAL PLATE CAPACITY Model Plates BACTRON300 (BACTRONII) 300 BACTRON600 (BACTRONIV) 600 BACTRON900 (B

64 | P a g e PARTS LIST Description Parts Number Anaerobic Indicator Strips (box of 100 packets) 9900706 Pass Box Door Gasket 12 x 12 (burgundy) 34

65 | P a g e PARTS (CONTINUED) ORDERING PARTS AND CONSUMABLES If you have the Part Number for an item, you may order it directly from Sheldon Man

66 | P a g e ACCESORIES Shel Lab offers the following BACTRON accessories for sale. Please see our website for prices. Acrylic Glass Cleaner (

67 | P a g e ACCESSORIES (CONTINUED) CAT 180 Bake Catalyst Oven A small, slide-loading desktop oven designed specifically for baking out BACTRON

68 | P a g e ACCESSORIES (CONTINUED) BACTRON600/900 Stand A rolling stand with two (2) cabinets for the BACTRON600 and 900. 29 inches high by 88 i

69 | P a g e ACCESSORIES (CONTINUED) UV Viewing Lamp A handheld UV lamp for use with BACTRON workstations. Parts Number 9490507 Zephiran Benzalk

7 | P a g e RECEIVING YOUR BACTRON Before leaving our factory, all BACTRONs are packaged in high-quality shipping materials to provide protection f

8 | P a g e RECEIVING YOUR BACTRON (CONTINUED) Carefully check all packaging before discarding. Save the shipping carton until you are certain that

9 | P a g e GRAPHIC SYMBOLS The BACTRON is provided with multiple graphic symbols located on its exterior and interior surfaces. The symbols identi